《植物生理学报》 2010, 46(4): 399-402

叶绿素含量测定的简化

舒展, 张晓素, 陈娟, 陈根云, 许大全*

中国科学院上海生命科学研究院植物生理生态研究所, 上海200032

通信作者：许大全；E-mail: dqxu@sippe.ac.cn；Tel: 021-54924231

摘　要：

本文介绍一种避免称重、研磨、冲洗和过滤等繁琐步骤, 仅将确定面积的叶片细丝用少量80%丙酮浸泡提取后便可 比色计算的简便的测定叶绿素含量的方法。此法省时间、省溶剂, 不受叶片含水量变化的干扰, 适合于大批量叶片样品的 测定, 而且便于与以单位叶面积表示的光合速率联系起来分析实验结果。同时, 指出一些可能影响测定结果的因素。

关键词：叶绿素含量; 叶绿素a/b; 叶片鲜重; 叶面积

收稿：2009-12-22   修定：2010-01-22

资助：国家重点基础研究发展规划项目(2005cb121106)。

The Simplification of Chlorophyll Content Measurement

SHU Zhan, ZHANG Xiao-Su, CHEN Juan, CHEN Gen-Yun, XU Da-Quan*

Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China

Corresponding author: XU Da-Quan; E-mail: dqxu@sippe.ac.cn; Tel: 021-54924231

Abstract:

A simple method of chlorophyll content measurement is introduced in the present paper. Namely, leaf filaments with known area are soaked in 80% (V/V) acetone until color of the filaments changed from green to white, and then the supernatant of extract is taken for light absorption measurement. This method elides some time-consuming steps such as determining of leaf fresh weight, grinding, washing, filtering and so on. It not only can save time and solvent, and avoid interference by changes in leaf water content, but also is more convenient to measurement of large amount samples, and analysis of measurement results with photosynthetic rate on the basis of leaf area. Moreover, some factors that may influence the result of chlorophyll content measurement are pointed out.

Key words: chlorophyll content; chlorophyll a/b; leaf fresh weight; leaf area